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Dionex CarboPac PA100 糖類分析色譜柱

更新時間：2019-11-12

簡要描述：

Dionex CarboPac PA100 糖類分析色譜柱
用于從糖蛋白中切下的低聚糖、中性和有唾液酸的N-連接低聚糖

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Dionex CarboPac PA100 糖類分析色譜柱

用途和特點：
CarboPac PA100用于從糖蛋白中切下的低聚糖、中性和有唾液酸的N-連接低聚糖；單糖連接多聚糖的異構(gòu)體；線性多聚糖剖面等的分離。

 

Dionex CarboPac PA100 糖類分析色譜柱

訂貨信息：

Dionex CarboPac PA100 糖類分析柱

分析柱
CarboPac PA100 Analytical Column (2 x 250 mm)057182
CarboPac PA100 Analytical Column (4 x 250 mm)043055
CarboPac PA100 Column (9 x 250 mm)SP2089
CarboPac PA100 Column (22 x 250 mm)SP2667
保護柱
CarboPac PA100 Guard Column (2 x 50 mm)057183
CarboPac PA100 Guard Column (4 x 50 mm)043054

 

 

Low-Picomole Oligosaccharide Analysis

Oligosaccharides released from therapeutic glycoproteins can differ in size, charge, branching, and linkage. Oligosaccharides with these features can be resolved using the CarboPac? PA100. It provides the flexibility to separate oligosaccharides into broad classes depending upon the degree of sialylation, while maintaining the resolution needed for routine analysis based on linkage isomerism. Coupled with pulsed amperometric detection, the PA100 supports detection at low-picomole levels.

  • Separation of closely related oligosaccharides, even isomers, at picomole levels

  • Separation of neutral and charged oligosaccharides in the same run

  • No sample derivatization required for detection

The CarboPac PA100 column is a high-resolution, strong anion-exchange column for enhanced chromatography of oligosaccharides.

The Carbohydrate Membrane Desalter can be used on-line prior to fraction collection.

 

CarboPac PA100 Column for Oligosaccharide Analysis Specifications
DimensionsCarboPac PA100 Analytical Column:
4 × 250 mm
CarboPac PA100 Guard Column:
4 × 50 mm
CarboPac PA100 Microbore Column:
2 × 250 mm
CarboPac PA100 Microbore Guard Column:
2 × 50 mm
CarboPac PA100 Semipreparative Column:
9 × 250 mm
CarboPac PA100 Semipreparative Column:
22 × 250 mm
Maximum operating pressure4000 psi (27.9 MPa)
Temperature range4–60 °C
Recommended operating temperature: ambient
Typical operating conditions2400 psi at 1.0 mL/min (analytical and guard columns)
Recommended flow rate: 1.0 mL/min
Mobile phase compatibilitypH 0–14; 100% compatible with common organic solvents.
Ionic form eluents: sodium acetate and sodium hydroxide only.
Resin composition8.5-μm-diameter ethylvinylbenzene/divinylbenzene substrate (55% cross-linking) agglomerated with 275-nm MicroBead? quaternary amine functionalized latex (6% cross-linked).
Anion-exchange capacityApproximately 90 μeq/column (4 × 250 mm analytical column)
Column constructionPEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.

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